Detection of Hepatitis B Virus Genome with Mutations Outside the Major Hydrophilic Region in the Surface Antigen Isolated from Patients with Coexisting HBsAg and Anti-HBs

Background: The leading cause of mutations in the hepatitis B virus (HBV) genome is high rate nucleotide misincorporation during reverse transcription. Most were found within “a” determinant S gene’s major hydrophilic region (MHR). They resulted escape mutants due to amino acid changes MHR. However, outside MHR can also trigger mutants. Objectives: This study focused on further molecular studies genotype D HBV DNA isolated from patients with chronic infection, together coexistence surface antigen (HBsAg) and antibodies (anti-HBs) their serum samples. Methods: In this study, samples 83 infection analyzed by serological immunological tests for concurrence HBsAg anti-HBs. addition, mutation was assessed sequencing genes within, upstream, downstream Results: Among anti-HBs detected 11 (13.25%) individuals. Mutations eight acids seven observed at 27 different sites three locations, namely affected structure epitope appearance an mutant. Conclusions: results indicated that upstream play a role infection.